Journal: Biomedicines
Article Title: Unraveling Microviscosity Changes Induced in Cancer Cells by Photodynamic Therapy with Targeted Genetically Encoded Photosensitizer
doi: 10.3390/biomedicines12112550
Figure Lengend Snippet: Plasma membrane microviscosity in HeLa tumor spheroids after PDT with KillerRed localized in the nuclei (H2B) or within the plasma membrane (PM). ( A ) Schematic representation of the spheroid area (shown by the yellow square) imaged by FLIM. The spheroid had adhered to the glass bottom, and the images were acquired from a depth of ~30 μm. Higher-magnification image of the molecular rotor distribution in spheroid cell membranes indicated by the red squares. The scale bar is 80 μm. ( B ) FLIM images and live/dead (LD) assay of control and treated cells in spheroids. Bar = 80 μm. ( C ) Morphology of control and treated spheroids. The scale bar is 80 μm. ( D ) Quantification of membrane microviscosity of spheroid cells after PDT. Means ± SD, n = 4 spheroids, 60 cells in each. ( E ) Quantitative analysis of dead cells in control and treated cell populations, %. * p < 0.05 with control; # p < 0.05 with KillerRed-H2B. CNT KR: control with different localization of KillerRed. H2B: cells with nuclear localization of KillerRed. PM: cells with membrane localization of KillerRed.
Article Snippet: For viscous imaging, a LSM 880 laser scanning microscope (Carl Zeiss, Gottingen, Germany) equipped with a FLIM SPC 150 TCSPC module (Becker & Hickl GmbH, Berlin, Germany) and a Mai Tai HP femtosecond laser (80 MHz, 140 fs, Spectra Physics, Milpitas, CA, USA) were used.
Techniques: Membrane, Control